CryoSPARC Discuss

Topic	Replies	Views	Activity
Before You Post: Troubleshooting Guidelines Troubleshooting Dear cryoSPARC users, Thank you for communicating with us on the discussion forum. Before you post your topic, check the following items: Are you on the latest version of cryoSPARC? cryosparcm update and see Softwar…	0	12874	October 15, 2018
Encountered a Closed Topic? Troubleshooting Forum moderators may Close topics for various reasons. If you find that a topic to which you would like to add a post has been Closed, please Create a new topic for your post. In your post, include the URL of the topic…	0	1921	November 30, 2023
Local resolution estimation issue Troubleshooting	0	14	September 11, 2026
How Can I reduce micelle-dominated alignment when applying HR-HAIR to a small GPCR? Cryo-EM Data Processing	6	166	September 11, 2026
NU refine resolution-5.5 A, map doesn't have those features 3D Reconstruction	4	86	September 11, 2026
Balance half sets Utilities	7	128	September 11, 2026
Losing ~30% of particles between 3D Classification and downstream jobs (Extraction/Reconstruction/Local Refine) — combined particle count doesn't match 3D Classification	0	23	September 11, 2026
ZFS pool strategy for CryoSPARC Hardware and Performance	1	59	September 11, 2026
Cluster Jobs SSD Cache Competition Troubleshooting	0	20	September 10, 2026
Bug in cloning session + template picker? CryoSPARC Live	0	21	September 9, 2026
FSC-work/FSC-free gap, and curves not converging near FSC=0.143 Troubleshooting	0	44	September 9, 2026
Resolving smeary density for a protein complex dataset Cryo-EM Data Processing	0	61	September 9, 2026
CTF estimation/CTFfind4 error CTF Estimation	1	37	September 9, 2026
Problem with topaz pick- test average precision is 0.1- picking false positives? Particle Picking	0	28	September 9, 2026
Unable to kill job / zombie job V5.0.7 Troubleshooting	6	89	September 7, 2026
5.0.6: ab-initio reconstruction loop 3D Reconstruction	33	538	September 7, 2026
Display bug when one class is empty in heterogeneous refinement Application	1	69	September 4, 2026
CryoSPARC-5.0.6 vs CryoSPARC 4.6.2 resolution changes for non uniform refinement 3D Refinement	2	104	September 4, 2026
SIGSEGV in core/ioengine/core.so across multiple job types Troubleshooting	5	86	September 4, 2026
Problem with .MRC file extension Motion Correction	0	44	September 4, 2026
Extraction box parameter in RBMC Motion Correction feature_request	0	47	September 4, 2026
Corrupted project json files after faulty reattaching Data Management	16	415	September 3, 2026
Reference model based ab-initio reconstruction 3D Reconstruction	8	4457	September 3, 2026
Changing interval for retrying saving results to disk post-5.0.7 Install, Configure and Update	3	34	September 2, 2026
Inspect Picks job custom polygon request Particle Curation feature_request	1	52	September 2, 2026
The resolution of the big protein is low 2D Classification	2	110	September 2, 2026
V5.0.7 – Instance Testing / Benchmark job fails with AttributeError: 'str' object has no attribute 'stat' when benchmark data is missing Troubleshooting	4	45	September 1, 2026
Heterogeneous reconstruction of small protein 3D Reconstruction	16	445	September 1, 2026
RTX PRO 4000 Blackwell Xid 8 / CUDA_ERROR_LAUNCH_TIMEOUT in 2D Classification and Ab-initio Troubleshooting	9	151	September 1, 2026
How to process highly flexible 150 kDa protein complex? Cryo-EM Data Processing	6	244	September 1, 2026

Before You Post: Troubleshooting Guidelines

Dear cryoSPARC users, Thank you for communicating with us on the discussion forum. Before you post your topic, check the following items: Are you on the latest version of cryoSPARC? cryosparcm update and see Softwar…

0

12874

October 15, 2018

Encountered a Closed Topic?

Troubleshooting

Forum moderators may Close topics for various reasons. If you find that a topic to which you would like to add a post has been Closed, please Create a new topic for your post. In your post, include the URL of the topic…