CryoSPARC Discuss

Topic	Replies	Views	Activity
Before You Post: Troubleshooting Guidelines Troubleshooting Dear cryoSPARC users, Thank you for communicating with us on the discussion forum. Before you post your topic, check the following items: Are you on the latest version of cryoSPARC? cryosparcm update and see Softwar…	0	12883	October 15, 2018
Encountered a Closed Topic? Troubleshooting Forum moderators may Close topics for various reasons. If you find that a topic to which you would like to add a post has been Closed, please Create a new topic for your post. In your post, include the URL of the topic…	0	1924	November 30, 2023
Unable to kill job / zombie job V5.0.7 Troubleshooting	8	105	September 16, 2026
Protein denaturation during freezing sample preparation Cryo-EM Data Processing	2	87	September 16, 2026
Local resolution estimation issue Post Processing	4	106	September 15, 2026
V5.0.7 project stuck in failed import state after historical DB-loss recovery Troubleshooting	0	13	September 15, 2026
Losing ~30% of particles between 3D Classification and downstream jobs (Extraction/Reconstruction/Local Refine) — combined particle count doesn't match 3D Classification	3	58	September 15, 2026
Topaz overwhelmed by mic input? Particle Picking	1	55	September 15, 2026
NU refine resolution-5.5 A, map doesn't have those features 3D Reconstruction	9	194	September 15, 2026
Bug in cloning session + template picker? CryoSPARC Live	1	38	September 14, 2026
Reference-based motion correction: "operands could not be broadcast together" Motion Correction	4	88	September 14, 2026
Tracking processing steps leading to the "final" map in CSV export Features and Functionality feature_request	0	28	September 14, 2026
CTF estimation/CTFfind4 error CTF Estimation	3	66	September 14, 2026
ZFS pool strategy for CryoSPARC Hardware and Performance	2	96	September 14, 2026
Using 3D Flex to refine a small, unrestricted domain 3D Flexible Refinement	6	602	September 13, 2026
How Can I reduce micelle-dominated alignment when applying HR-HAIR to a small GPCR? Cryo-EM Data Processing	6	188	September 11, 2026
Balance half sets Utilities	7	142	September 11, 2026
Cluster Jobs SSD Cache Competition Troubleshooting	0	28	September 10, 2026
FSC-work/FSC-free gap, and curves not converging near FSC=0.143 Troubleshooting	0	54	September 9, 2026
Resolving smeary density for a protein complex dataset Cryo-EM Data Processing	0	77	September 9, 2026
Problem with topaz pick- test average precision is 0.1- picking false positives? Particle Picking	0	39	September 9, 2026
5.0.6: ab-initio reconstruction loop 3D Reconstruction	33	548	September 7, 2026
Display bug when one class is empty in heterogeneous refinement Application	1	75	September 4, 2026
CryoSPARC-5.0.6 vs CryoSPARC 4.6.2 resolution changes for non uniform refinement 3D Refinement	2	114	September 4, 2026
SIGSEGV in core/ioengine/core.so across multiple job types Troubleshooting	5	92	September 4, 2026
Problem with .MRC file extension Motion Correction	0	49	September 4, 2026
Extraction box parameter in RBMC Motion Correction feature_request	0	52	September 4, 2026
Corrupted project json files after faulty reattaching Data Management	16	421	September 3, 2026
Reference model based ab-initio reconstruction 3D Reconstruction	8	4473	September 3, 2026
Changing interval for retrying saving results to disk post-5.0.7 Install, Configure and Update	3	38	September 2, 2026

Before You Post: Troubleshooting Guidelines

Dear cryoSPARC users, Thank you for communicating with us on the discussion forum. Before you post your topic, check the following items: Are you on the latest version of cryoSPARC? cryosparcm update and see Softwar…

0

12883

October 15, 2018

Encountered a Closed Topic?

Troubleshooting

Forum moderators may Close topics for various reasons. If you find that a topic to which you would like to add a post has been Closed, please Create a new topic for your post. In your post, include the URL of the topic…