Report and troubleshoot errors encountered when processing data.
How to install, configure and update cryoSPARC on a workstation or cluster. Please refer to these instructions first.
Requests for new jobs and features in cryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, CryoSPARC Parameters, etc.).
Questions, discussion and errors encountered when performing global (rigid) and local (per particle) motion correction.
Questions and discussion regarding the 3D variability analysis job.
Questions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes and other files.
Questions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.
Questions, discussion and error messages arising when curating and inspecting micrographs.
Questions, discussion and errors encountered when estimating CTF values.
Questions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis. Also for utilities such as volume tools and sharpening.
Questions and discussion relating to hardware setup and cryoSPARC performance.
Questions about job parameters and how to optimize these.
Best practices and how-tos for using and getting the most out of cryoSPARC.
Questions, discussion and errors encountered when using local refinement and signal subtraction.
Questions, discussion and error messages relating to manual and template based automatic particle picking, and particle extraction.
Questions, discussion and error messages relating to 2D classification of particles.
Questions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.
General questions and discussion relating to cryo-EM image processing and methods.