Category | Topics |
---|---|
Install, Configure and UpdateHow to install, configure and update cryoSPARC on a workstation or cluster. Please refer to these instructions first.
|
1037
|
CTF RefinementQuestions and issues related to CTF refinement (global and per-particle).
|
46
|
TroubleshootingReport and troubleshoot errors encountered when processing data. Please be as specific as possible and follow the Troubleshooting Guidelines.
|
1424
|
CryoSPARC ParametersQuestions about job parameters and how to optimize these.
|
17
|
Particle PickingQuestions, discussion and error messages relating to particle picking (manual, blob-based, template-based, deep picking), particle extraction and inspection.
|
303
|
Helical ProcessingQuestions relating to helical processing.
|
80
|
3D Variability AnalysisQuestions and discussion regarding the 3D variability analysis and 3D variability display.
|
126
|
CTF EstimationQuestions, discussion and errors encountered when estimating CTF values.
|
66
|
Motion CorrectionQuestions, discussion and errors encountered when performing patch-based, global and local motion correction.
|
277
|
ApplicationTopics related to the CryoSPARC web application/user interface.
|
179
|
Features and FunctionalityRequests for new jobs and features in cryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, CryoSPARC Parameters, etc.).
|
545
|
Local RefinementQuestions, discussion and errors encountered when using local refinement and signal subtraction.
|
140
|
46
|
|
Cryo-EM Data ProcessingGeneral questions and discussion relating to cryo-EM image processing and methods.
|
126
|
3D ClassificationQuestions and discussion regarding 3D Classification.
|
89
|
Import and ExportQuestions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes and other files.
|
454
|
CryoSPARC LiveQuestions, issues and feature requests relating to cryoSPARC Live.
|
163
|
3D ReconstructionQuestions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.
|
114
|
Hardware and PerformanceQuestions and discussion relating to hardware setup and cryoSPARC performance.
|
82
|
2D ClassificationQuestions, discussion and error messages relating to 2D classification of particles.
|
188
|
Post ProcessingQuestions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis.
|
47
|
Data ManagementQuestions and discussion relating to data management for cryo-EM.
|
106
|
3D RefinementQuestions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.
|
351
|
UtilitiesFor discussion about Volume Tools, Sharpening Tools, Particle Sets Tools, Exposure Sets Tools, etc.
|
88
|
Exposure CurationQuestions, discussion and error messages arising when curating and inspecting micrographs.
|
46
|
Data Processing StrategyQuestions and advice on data processing strategy for particular samples or datasets. Discussion and best practices/tips are welcome!
|
20
|
TipsBest practices and how-tos for using and getting the most out of cryoSPARC.
|
150
|
3D Flexible RefinementQuestions and Discussion regarding 3DFlex jobs (Data Prep, Mesh Prep, Training, Generator, Reconstruction)
|
102
|
Cryo-EM Sample PreparationTopics related to the preparation of samples for cryogenic electron microscopy.
|
11
|
Model BuildingQuestions and discussions around the building, refinement and validation of molecular models based on cryo-EM data.
|
11
|
ScriptingScripting in or with CryoSPARC: CryoSPARC CLI, cryosparc-tools, etc.
|
36
|
Workshops & ConferencesAnnouncements about cryo-EM workshops and conferences that may be of general interest to users.
|
1
|