Category Topics

3D Refinement

Questions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.
109

Features and Functionality

Requests for new jobs and features in cryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, CryoSPARC Parameters, etc.).
333
16

Particle Picking

Questions, discussion and error messages relating to manual and template based automatic particle picking, and particle extraction.
64

Import and Export

Questions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes and other files.
167

Troubleshooting

Report and troubleshoot errors encountered when processing data.
884

CryoSPARC Live

Questions, issues and feature requests relating to cryoSPARC Live.
41

Install, Configure and Update

How to install, configure and update cryoSPARC on a workstation or cluster. Please refer to these instructions first.
340

CryoSPARC Parameters

Questions about job parameters and how to optimize these.
13
17

Local Refinement

Questions, discussion and errors encountered when using local refinement and signal subtraction.
48

3D Reconstruction

Questions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.
25

3D Variability Analysis

Questions and discussion regarding the 3D variability analysis job.
50

Tips

Best practices and how-tos for using and getting the most out of cryoSPARC.
114

Motion Correction

Questions, discussion and errors encountered when performing global (rigid) and local (per particle) motion correction.
72

2D Classification

Questions, discussion and error messages relating to 2D classification of particles.
53

Post Processing

Questions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis. Also for utilities such as volume tools and sharpening.
8

Exposure Curation

Questions, discussion and error messages arising when curating and inspecting micrographs.
22

Cryo-EM Data Processing

General questions and discussion relating to cryo-EM image processing and methods.
38

CTF Estimation

Questions, discussion and errors encountered when estimating CTF values.
22
2

Utilities

For discussion about Volume Tools, Sharpening Tools, Particle Sets Tools, Exposure Sets Tools.
8

Data Management

Questions and discussion relating to data management for cryo-EM.
12

Hardware and Performance

Questions and discussion relating to hardware setup and cryoSPARC performance.
40