| Category | Topics |
|---|---|
3D RefinementQuestions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.
|
109
|
Features and FunctionalityRequests for new jobs and features in cryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, CryoSPARC Parameters, etc.).
|
333
|
|
16
|
|
Particle PickingQuestions, discussion and error messages relating to manual and template based automatic particle picking, and particle extraction.
|
64
|
Import and ExportQuestions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes and other files.
|
167
|
TroubleshootingReport and troubleshoot errors encountered when processing data.
|
884
|
CryoSPARC LiveQuestions, issues and feature requests relating to cryoSPARC Live.
|
41
|
Install, Configure and UpdateHow to install, configure and update cryoSPARC on a workstation or cluster. Please refer to these instructions first.
|
340
|
CryoSPARC ParametersQuestions about job parameters and how to optimize these.
|
13
|
|
17
|
|
Local RefinementQuestions, discussion and errors encountered when using local refinement and signal subtraction.
|
48
|
3D ReconstructionQuestions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.
|
25
|
3D Variability AnalysisQuestions and discussion regarding the 3D variability analysis job.
|
50
|
TipsBest practices and how-tos for using and getting the most out of cryoSPARC.
|
114
|
Motion CorrectionQuestions, discussion and errors encountered when performing global (rigid) and local (per particle) motion correction.
|
72
|
2D ClassificationQuestions, discussion and error messages relating to 2D classification of particles.
|
53
|
Post ProcessingQuestions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis. Also for utilities such as volume tools and sharpening.
|
8
|
Exposure CurationQuestions, discussion and error messages arising when curating and inspecting micrographs.
|
22
|
Cryo-EM Data ProcessingGeneral questions and discussion relating to cryo-EM image processing and methods.
|
38
|
CTF EstimationQuestions, discussion and errors encountered when estimating CTF values.
|
22
|
|
2
|
|
UtilitiesFor discussion about Volume Tools, Sharpening Tools, Particle Sets Tools, Exposure Sets Tools.
|
8
|
Data ManagementQuestions and discussion relating to data management for cryo-EM.
|
12
|
Hardware and PerformanceQuestions and discussion relating to hardware setup and cryoSPARC performance.
|
40
|
