Category | Topics |
---|---|
Particle PickingQuestions, discussion and error messages relating to particle picking (manual, blob-based, template-based, deep picking), particle extraction and inspection.
|
284
|
2D ClassificationQuestions, discussion and error messages relating to 2D classification of particles.
|
179
|
Install, Configure and UpdateHow to install, configure and update cryoSPARC on a workstation or cluster. Please refer to these instructions first.
|
1003
|
Features and FunctionalityRequests for new jobs and features in cryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, CryoSPARC Parameters, etc.).
|
542
|
3D Flexible RefinementQuestions and Discussion regarding 3DFlex jobs (Data Prep, Mesh Prep, Training, Generator, Reconstruction)
|
97
|
Data ManagementQuestions and discussion relating to data management for cryo-EM.
|
103
|
TroubleshootingReport and troubleshoot errors encountered when processing data. Please be as specific as possible and follow the Troubleshooting Guidelines.
|
1408
|
Import and ExportQuestions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes and other files.
|
442
|
CTF RefinementQuestions and issues related to CTF refinement (global and per-particle).
|
44
|
Cryo-EM Data ProcessingGeneral questions and discussion relating to cryo-EM image processing and methods.
|
121
|
Post ProcessingQuestions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis.
|
42
|
3D ClassificationQuestions and discussion regarding 3D Classification.
|
80
|
Helical ProcessingQuestions relating to helical processing.
|
76
|
3D ReconstructionQuestions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.
|
107
|
ApplicationTopics related to the CryoSPARC web application/user interface.
|
165
|
3D Variability AnalysisQuestions and discussion regarding the 3D variability analysis and 3D variability display.
|
121
|
3D RefinementQuestions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.
|
338
|
Cryo-EM Sample PreparationTopics related to the preparation of samples for cryogenic electron microscopy.
|
10
|
Motion CorrectionQuestions, discussion and errors encountered when performing patch-based, global and local motion correction.
|
268
|
CryoSPARC LiveQuestions, issues and feature requests relating to cryoSPARC Live.
|
156
|
CTF EstimationQuestions, discussion and errors encountered when estimating CTF values.
|
64
|
UtilitiesFor discussion about Volume Tools, Sharpening Tools, Particle Sets Tools, Exposure Sets Tools, etc.
|
83
|
Local RefinementQuestions, discussion and errors encountered when using local refinement and signal subtraction.
|
127
|
Model BuildingQuestions and discussions around the building, refinement and validation of molecular models based on cryo-EM data.
|
11
|
ScriptingScripting in or with CryoSPARC: CryoSPARC CLI, cryosparc-tools, etc.
|
36
|
Workshops & ConferencesAnnouncements about cryo-EM workshops and conferences that may be of general interest to users.
|
1
|
TipsBest practices and how-tos for using and getting the most out of cryoSPARC.
|
149
|
Data Processing StrategyQuestions and advice on data processing strategy for particular samples or datasets. Discussion and best practices/tips are welcome!
|
15
|
CryoSPARC ParametersQuestions about job parameters and how to optimize these.
|
17
|
Exposure CurationQuestions, discussion and error messages arising when curating and inspecting micrographs.
|
41
|
43
|
|
Hardware and PerformanceQuestions and discussion relating to hardware setup and cryoSPARC performance.
|
79
|