How to install, configure and update CryoSPARC on a workstation or cluster. Please refer to: https://guide.cryosparc.com/setup-configuration-and-management/how-to-download-install-and-configure

Report and troubleshoot errors encountered when processing data. Please be as specific as possible and follow the Troubleshooting Guidelines.

Topics related to the CryoSPARC web application/user interface.

Requests for new jobs and features in CryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, 3D refinement, etc.)

Questions and discussion relating to data management for cryo-EM.

Scripting in or with CryoSPARC: CryoSPARC CLI, cryosparc-tools, etc.

Questions and discussion relating to hardware setup and CryoSPARC performance.

Questions, discussion and educational topics relating to cryo-EM image processing and computational methods, along with best practices and tips for data processing.

Questions, issues and feature requests relating to CryoSPARC Live.

Questions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes, metadata and other files.

Questions, discussion and errors encountered when performing patch-based, global and local motion correction.

Questions, discussion and errors encountered when estimating CTF values.

Questions, discussion and error messages arising when curating and inspecting micrographs.

Questions, discussion and error messages relating to particle picking (manual, blob-based, template-based, deep picking), particle extraction and inspection.

Questions, discussion and error messages relating to 2D classification of particles.

Questions and discussion regarding curation of a particle stack, from Inspect Picks to 2D and 3D classification methods.

Questions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.

Questions and discussion regarding 3D Classification.

Questions and discussion regarding the 3D variability analysis and 3D variability display.

Questions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.

Questions and issues related to CTF refinement (global and per-particle).

Questions and Discussion regarding 3DFlex jobs (Data Prep, Mesh Prep, Training, Generator, Reconstruction)

Questions, discussion and errors encountered when using local refinement and signal subtraction.

Questions relating to helical processing.

Questions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis.

For discussion about Volume Tools, Sharpening Tools, Particle Sets Tools, Exposure Sets Tools, etc.

Topics related to the preparation of samples for cryogenic electron microscopy.

Questions and discussions around the building, refinement and validation of molecular models based on cryo-EM data.

Announcements about cryo-EM workshops and conferences that may be of general interest to users.