Category | Topics |
---|---|
TroubleshootingReport and troubleshoot errors encountered when processing data. Please be as specific as possible and follow the Troubleshooting Guidelines.
|
1274
|
Cryo-EM Data ProcessingGeneral questions and discussion relating to cryo-EM image processing and methods.
|
80
|
36
|
|
Data Processing StrategyQuestions and advice on data processing strategy for particular samples or datasets. Discussion and best practices/tips are welcome!
|
8
|
Install, Configure and UpdateHow to install, configure and update cryoSPARC on a workstation or cluster. Please refer to these instructions first.
|
764
|
Data ManagementQuestions and discussion relating to data management for cryo-EM.
|
63
|
Features and FunctionalityRequests for new jobs and features in cryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, CryoSPARC Parameters, etc.).
|
508
|
CTF EstimationQuestions, discussion and errors encountered when estimating CTF values.
|
55
|
ApplicationTopics related to the CryoSPARC web application/user interface.
|
115
|
2D ClassificationQuestions, discussion and error messages relating to 2D classification of particles.
|
138
|
Exposure CurationQuestions, discussion and error messages arising when curating and inspecting micrographs.
|
32
|
3D ReconstructionQuestions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.
|
68
|
3D Flexible RefinementQuestions and Discussion regarding 3DFlex jobs (Data Prep, Mesh Prep, Training, Generator, Reconstruction)
|
66
|
Particle PickingQuestions, discussion and error messages relating to particle picking (manual, blob-based, template-based, deep picking), particle extraction and inspection.
|
184
|
CryoSPARC LiveQuestions, issues and feature requests relating to cryoSPARC Live.
|
112
|
ScriptingScripting in or with CryoSPARC: CryoSPARC CLI, cryosparc-tools, etc.
|
17
|
Helical ProcessingQuestions relating to helical processing.
|
53
|
3D ClassificationQuestions and discussion regarding 3D Classification.
|
47
|
Motion CorrectionQuestions, discussion and errors encountered when performing patch-based, global and local motion correction.
|
149
|
3D Variability AnalysisQuestions and discussion regarding the 3D variability analysis and 3D variability display.
|
106
|
Import and ExportQuestions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes and other files.
|
344
|
TipsBest practices and how-tos for using and getting the most out of cryoSPARC.
|
139
|
3D RefinementQuestions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.
|
252
|
CTF RefinementQuestions and issues related to CTF refinement (global and per-particle).
|
37
|
Local RefinementQuestions, discussion and errors encountered when using local refinement and signal subtraction.
|
104
|
Hardware and PerformanceQuestions and discussion relating to hardware setup and cryoSPARC performance.
|
69
|
Post ProcessingQuestions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis.
|
30
|
Model BuildingQuestions and discussions around the building, refinement and validation of molecular models based on cryo-EM data.
|
6
|
CryoSPARC ParametersQuestions about job parameters and how to optimize these.
|
15
|
UtilitiesFor discussion about Volume Tools, Sharpening Tools, Particle Sets Tools, Exposure Sets Tools, etc.
|
48
|
Cryo-EM Sample PreparationTopics related to the preparation of samples for cryogenic electron microscopy.
|
5
|