Questions and discussion regarding the 3D variability analysis and 3D variability display.

Questions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.

How to install, configure and update cryoSPARC on a workstation or cluster. Please refer to these instructions first.

Report and troubleshoot errors encountered when processing data. Please be as specific as possible and follow the Troubleshooting Guidelines.

Questions and discussion regarding 3D Classification.

Topics related to the CryoSPARC web application/user interface.

Requests for new jobs and features in cryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, CryoSPARC Parameters, etc.).

Questions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes and other files.

Questions, discussion and error messages relating to particle picking (manual, blob-based, template-based, deep picking), particle extraction and inspection.

Questions, issues and feature requests relating to cryoSPARC Live.

Questions, discussion and errors encountered when performing patch-based, global and local motion correction.

Questions and Discussion regarding 3DFlex jobs (Data Prep, Mesh Prep, Training, Generator, Reconstruction)

Questions, discussion and errors encountered when estimating CTF values.

Questions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.

Questions, discussion and error messages relating to 2D classification of particles.

For discussion about Volume Tools, Sharpening Tools, Particle Sets Tools, Exposure Sets Tools, etc.

General questions and discussion relating to cryo-EM image processing and methods.

Questions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis.

Questions, discussion and errors encountered when using local refinement and signal subtraction.

Questions about job parameters and how to optimize these.

Questions and discussion relating to data management for cryo-EM.

Questions and issues related to CTF refinement (global and per-particle).

Questions, discussion and error messages arising when curating and inspecting micrographs.

Questions and discussions around the building, refinement and validation of molecular models based on cryo-EM data.

Questions relating to helical processing.

Topics related to the preparation of samples for cryogenic electron microscopy.

Questions and advice on data processing strategy for particular samples or datasets. Discussion and best practices/tips are welcome!