Category Topics
12

Troubleshooting

Report and troubleshoot errors encountered when processing data. Please be as specific as possible and follow the Troubleshooting Guidelines.
1131

Features and Functionality

Requests for new jobs and features in cryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, CryoSPARC Parameters, etc.).
443

Install, Configure and Update

How to install, configure and update cryoSPARC on a workstation or cluster. Please refer to these instructions first.
503

Import and Export

Questions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes and other files.
263

3D Variability Analysis

Questions and discussion regarding the 3D variability analysis and 3D variability display.
89

Local Refinement

Questions, discussion and errors encountered when using local refinement and signal subtraction.
76

Particle Picking

Questions, discussion and error messages relating to particle picking (manual, blob-based, template-based, deep picking), particle extraction and inspection.
104

Cryo-EM Data Processing

General questions and discussion relating to cryo-EM image processing and methods.
60

3D Reconstruction

Questions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.
43

Motion Correction

Questions, discussion and errors encountered when performing patch-based, global and local motion correction.
109

2D Classification

Questions, discussion and error messages relating to 2D classification of particles.
83

Cryo-EM Sample Preparation

Topics related to the preparation of samples for cryogenic electron microscopy.
2

CTF Estimation

Questions, discussion and errors encountered when estimating CTF values.
33

Hardware and Performance

Questions and discussion relating to hardware setup and cryoSPARC performance.
59

CTF Refinement

Questions and issues related to CTF refinement (global and per-particle).
22

Data Management

Questions and discussion relating to data management for cryo-EM.
29

Helical Processing

Questions relating to helical processing.
37

Post Processing

Questions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis.
16

Tips

Best practices and how-tos for using and getting the most out of cryoSPARC.
127

Utilities

For discussion about Volume Tools, Sharpening Tools, Particle Sets Tools, Exposure Sets Tools, etc.
23

CryoSPARC Live

Questions, issues and feature requests relating to cryoSPARC Live.
80

3D Refinement

Questions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.
190

Exposure Curation

Questions, discussion and error messages arising when curating and inspecting micrographs.
24

3D Classification

Questions and discussion regarding 3D Classification.
16

Model Building

Questions and discussions around the building, refinement and validation of molecular models based on cryo-EM data.
1

Data Processing Strategy

Questions and advice on data processing strategy for particular samples or datasets. Discussion and best practices/tips are welcome!
4

CryoSPARC Parameters

Questions about job parameters and how to optimize these.
14