Category | Topics |
---|---|
CryoSPARC LiveQuestions, issues and feature requests relating to cryoSPARC Live.
|
149
|
Exposure CurationQuestions, discussion and error messages arising when curating and inspecting micrographs.
|
40
|
Data ManagementQuestions and discussion relating to data management for cryo-EM.
|
98
|
Install, Configure and UpdateHow to install, configure and update cryoSPARC on a workstation or cluster. Please refer to these instructions first.
|
955
|
Motion CorrectionQuestions, discussion and errors encountered when performing patch-based, global and local motion correction.
|
254
|
Particle PickingQuestions, discussion and error messages relating to particle picking (manual, blob-based, template-based, deep picking), particle extraction and inspection.
|
263
|
ScriptingScripting in or with CryoSPARC: CryoSPARC CLI, cryosparc-tools, etc.
|
33
|
TroubleshootingReport and troubleshoot errors encountered when processing data. Please be as specific as possible and follow the Troubleshooting Guidelines.
|
1372
|
3D RefinementQuestions, discussion and errors relating to homogeneous and heterogeneous (multi-reference) refinement, and non-uniform refinement.
|
323
|
3D ReconstructionQuestions, discussion and errors encountered when performing homogeneous and heterogeneous ab-initio reconstruction.
|
96
|
Import and ExportQuestions, discussion and errors encountered when importing and exporting movies, micrographs, particles, volumes and other files.
|
424
|
Cryo-EM Data ProcessingGeneral questions and discussion relating to cryo-EM image processing and methods.
|
110
|
3D Flexible RefinementQuestions and Discussion regarding 3DFlex jobs (Data Prep, Mesh Prep, Training, Generator, Reconstruction)
|
92
|
ApplicationTopics related to the CryoSPARC web application/user interface.
|
156
|
Data Processing StrategyQuestions and advice on data processing strategy for particular samples or datasets. Discussion and best practices/tips are welcome!
|
13
|
Helical ProcessingQuestions relating to helical processing.
|
71
|
UtilitiesFor discussion about Volume Tools, Sharpening Tools, Particle Sets Tools, Exposure Sets Tools, etc.
|
74
|
Local RefinementQuestions, discussion and errors encountered when using local refinement and signal subtraction.
|
126
|
Post ProcessingQuestions, discussion and errors relating to post processing job types, including local resolution estimation, local filtering, align 3D maps, reslog type analysis, and 3DFSC analysis.
|
40
|
Hardware and PerformanceQuestions and discussion relating to hardware setup and cryoSPARC performance.
|
80
|
Features and FunctionalityRequests for new jobs and features in cryoSPARC. If you have questions about an existing job or feature, please submit them under the appropriate category (e.g., 3D reconstruction, CryoSPARC Parameters, etc.).
|
539
|
TipsBest practices and how-tos for using and getting the most out of cryoSPARC.
|
146
|
42
|
|
CTF EstimationQuestions, discussion and errors encountered when estimating CTF values.
|
61
|
CTF RefinementQuestions and issues related to CTF refinement (global and per-particle).
|
42
|
2D ClassificationQuestions, discussion and error messages relating to 2D classification of particles.
|
173
|
Cryo-EM Sample PreparationTopics related to the preparation of samples for cryogenic electron microscopy.
|
8
|
Model BuildingQuestions and discussions around the building, refinement and validation of molecular models based on cryo-EM data.
|
9
|
3D ClassificationQuestions and discussion regarding 3D Classification.
|
74
|
3D Variability AnalysisQuestions and discussion regarding the 3D variability analysis and 3D variability display.
|
120
|
CryoSPARC ParametersQuestions about job parameters and how to optimize these.
|
17
|