Dear colleagues,
I am dealing with very small protein.
Could you please provide recommendations for image processing?
Currently, I am curious about the particle picking // 2D and 3D refinement.
Thank you.
Kind regards,
Dmitry
Dear colleagues,
I am dealing with very small protein.
Could you please provide recommendations for image processing?
Currently, I am curious about the particle picking // 2D and 3D refinement.
Thank you.
Kind regards,
Dmitry
Hi Dimitry,
Here is a tutorial for the processing of membrane proteins:
The settings explained there helped me with small non-membrane targets before. Alternatively, this issue has been discussed elsewhere. The approaches suggested in these threads also helped me to get a grip on smaller targets.
Cheers,
Lorenz
If you want to train with a positive control, we uploaded micrographs of 65 kDa hemoglobin that lead to a 3 ang map to EMPIAR a while back EMPIAR-10721 CryoEM single particle analysis of 65 kDa human haemoglobin using a 200 kV Talos Arctica
In our hands increasing the dose experimentally to 60-80 e/ang2 had higher impact than processing tricks