hi, error is below:
assert mic_shape == mic.shape, “Micrograph shapes are inconsistent!” # otherwise micrograph_blob/shape is inconsistent with the actual frame shape
AssertionError: Micrograph shapes are inconsistent!
I have tried this on v 4.4.0 and 4.2.1 and same error.
The data are negative stain, which we collected in tif format (jpeg). I had to convert this to no-compression tiff then used tif2mrc. In the end it did result in mrc files which could be imported into cryosparc, picking succeeds, but at the extraction step I received that error for about half the images. The half which worked I can continue processing in 2D classification etc. When I use “header” (imod program) they both seem identical in terms of dimensions and other things.
note that we did not do CTF estimation since, as my understanding is, it is basically pointless for stain data.
any help is appreciated