CryoSPARC Discuss

Topic	Replies	Views	Activity
Before You Post: Troubleshooting Guidelines Troubleshooting Dear cryoSPARC users, Thank you for communicating with us on the discussion forum. Before you post your topic, check the following items: Are you on the latest version of cryoSPARC? cryosparcm update and see Softwar…	0	12967	October 15, 2018
Encountered a Closed Topic? Troubleshooting Forum moderators may Close topics for various reasons. If you find that a topic to which you would like to add a post has been Closed, please Create a new topic for your post. In your post, include the URL of the topic…	0	1957	November 30, 2023
5.0.6: ab-initio reconstruction loop 3D Reconstruction	36	698	October 30, 2026
Troubleshooting the CryoSPARC Live File Engine CryoSPARC Live	5	105	October 29, 2026
Moving the whole database and data to a new cluster Data Management	2	32	October 29, 2026
Local resolution estimation issue Post Processing	5	221	October 29, 2026
CryoSPARC v5.0.7 – Persistent master–worker version mismatch despite matching revisions and forced worker reinstall Install, Configure and Update	3	35	October 29, 2026
Helix Refine with C2 symmtry? Helical Processing	4	87	October 29, 2026
Cache particles ==>3D classification cache false Application	0	31	October 27, 2026
3D classification job needs to remember the user defined threshold setting Application feature_request	6	140	October 26, 2026
Difficult particle alignment of an ABC transporter solubilized in CubiPol polymers Cryo-EM Data Processing	5	158	October 26, 2026
Detected NaN values during Cryosparc v5 negstain ab-ini 3D Reconstruction	7	121	October 25, 2026
5.0.7 Cryosparc live: Stuck in Restoration Failed CryoSPARC Live	6	83	October 24, 2026
Topaz overwhelmed by mic input? Particle Picking	3	146	October 23, 2026
Amyloid helical reconstruction in cryoSPARC: guidelines, benchmarks and a feature wishlist Helical Processing	4	196	October 23, 2026
Error while launching jobs - Job is in status launched so it cannot be run Troubleshooting	7	73	October 23, 2026
Difficulty Improving 3D Reconstruction Beyond 5–6 Å Cryo-EM Data Processing	3	238	October 22, 2026
Configuring Per-User SSD Cache for CryoSPARC Workers Connected Directly to the Master Install, Configure and Update	4	105	October 22, 2026
Hostname Mismatch Install, Configure and Update	7	83	October 22, 2026
Hugepages are causing Motion Correction to fail? Motion Correction	3	93	October 22, 2026
CPU allocation in live session CryoSPARC Live	1	55	October 22, 2026
Master node reboot procedure Install, Configure and Update	1	61	October 22, 2026
Setting 20 or fewer 2D classes enables marginalisation 2D Classification	3	175	October 22, 2026
FAILED TO LAUNCH ON WORKER NODE return code 255 Install, Configure and Update	6	82	October 22, 2026
Symmetry expansion, local refinement, and composite maps Local Refinement	0	65	October 21, 2026
Reassign Particles - Reprocessed Micrographs Cryo-EM Data Processing	11	388	October 20, 2026
Gpfs ssd scratch bad performance Hardware and Performance	7	179	October 20, 2026
Poor CV scores and strange dose weight plot for reference-based motion correction with low dose per frame Motion Correction	2	97	October 20, 2026
Small membrane protein with flexible fiducials and preferred orientation issue: Need help with particle-picking strategy? Particle Picking	3	161	October 19, 2026
CUDA_ERROR_OUT_OF_MEMORY in nonuniform refinement 3D Refinement	6	125	October 19, 2026

Before You Post: Troubleshooting Guidelines

Dear cryoSPARC users, Thank you for communicating with us on the discussion forum. Before you post your topic, check the following items: Are you on the latest version of cryoSPARC? cryosparcm update and see Softwar…

0

12967

October 15, 2018

Encountered a Closed Topic?

Troubleshooting

Forum moderators may Close topics for various reasons. If you find that a topic to which you would like to add a post has been Closed, please Create a new topic for your post. In your post, include the URL of the topic…