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Topaz Train OOM on A100 80GB with very small dataset (cryoSPARC 4.5.3， new installed Topaz 0.2.5, A100 GPU 80 GB))
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6
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127
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November 6, 2025
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RBMC - Strange FCC Model Fit
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6
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116
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November 6, 2025
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Multinode GPU jobs
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2
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70
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November 5, 2025
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Gathering info on HPC configs for transparent hugepages
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5
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118
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November 5, 2025
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Combining datasets with same pixel size for helical refinement
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3
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99
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November 5, 2025
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Topaz extraction -struct.error: unpack requires a buffer of 1024 bytes
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4
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421
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November 2, 2025
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Csparc2star conversion for re-extracted particles
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1
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72
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November 1, 2025
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Recenter and rebox after subtraction
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8
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1122
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October 31, 2025
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Topaz precision curve oscillates over epochs
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1
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55
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October 31, 2025
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GSFSC curves fall very fast
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2
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115
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October 31, 2025
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Reference-based selection: retrospective threshold determination
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0
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49
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October 30, 2025
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Best practices for aligning similar maps for deposition?
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18
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462
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October 30, 2025
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Poor patch CTF estimation resolution
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5
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95
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October 29, 2025
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Configuring workers to use a different master hostname
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19
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165
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October 29, 2025
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Dynamic reassembly of Card View
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2
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82
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October 29, 2025
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Map fragmentation in Homogeneous Refinement
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23
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227
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October 29, 2025
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Can particle stacks exported from cryoSPARC be used for further RELION refinement without re-extraction?
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4
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124
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October 28, 2025
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Remove Duplicates tuning integrated into Inspect Picks job
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2
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82
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October 28, 2025
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Select 2D - Job process terminated abnormally
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12
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2539
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October 28, 2025
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Generating very large composite maps: overcoming computational limitation and confirming my approach is valid
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0
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59
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October 28, 2025
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Data processing for crowded micrograph
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10
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224
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October 28, 2025
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Error while 2D classification:Encountered OS error while caching: [Errno 22] Invalid argument:
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4
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120
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October 27, 2025
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Does cryosparc remove duplicate particles?
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12
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3905
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October 27, 2025
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Issue with 3D Variability Analysis in cryoSPARC
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6
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118
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October 27, 2025
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Particles Show No Motion Trajectories After Reference-Based Motion Correction
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5
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91
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October 25, 2025
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Workflow -> multiple queue to lane options (job dependent)
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2
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76
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October 24, 2025
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2D classification showing two bright dots at particle ends (protein–RNA complex ~200 kDa)
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0
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77
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October 24, 2025
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Problem with 150 kDa protein complex cryo-EM data processing
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2
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172
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October 24, 2025
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Reassign Particles - Reprocessed Micrographs
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6
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130
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October 24, 2025
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Purpose of exposure group curation with regex in End-to_end automation workflows
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3
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66
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October 24, 2025
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