Cryo-EM Data Processing

Topic	Replies	Views	Activity
Case Study: End-to-end and exploratory processing of a motor-bound nucleosome (EMPIAR-10739) education_and_advice Hi CryoSPARC users! We are excited to share with you a new end-to-end processing case study of a nucleosome with a motor protein bound that you can follow along with! Here we guide you all the way from downloading an EM…	1	99	October 30, 2025
About the Cryo-EM Data Processing category	0	837	September 17, 2018
Re-extract particles in Relion from CryoSPARC	21	140	November 6, 2025
Best practices for aligning similar maps for deposition?	18	421	October 30, 2025
Map fragmentation in Homogeneous Refinement	23	194	October 29, 2025
Generating very large composite maps: overcoming computational limitation and confirming my approach is valid	0	47	October 28, 2025
Data processing for crowded micrograph	10	182	October 28, 2025
2D classification showing two bright dots at particle ends (protein–RNA complex ~200 kDa)	0	66	October 24, 2025
Problem with 150 kDa protein complex cryo-EM data processing	2	151	October 24, 2025
Reassign Particles - Reprocessed Micrographs	6	121	October 24, 2025
Random seed behavior feature_request	3	93	October 22, 2025
Data processing on tilted and untilted data	2	70	October 20, 2025
Trouble fitting a complex into <4 Å map - severe clashes at interface despite tilted dataset	3	106	October 18, 2025
Manually defined dose-weights?	5	397	October 16, 2025
How to extract a 3D density volume slice in reciprocal space	0	27	October 16, 2025
Unusual Viewing Direction Distribution in high resolution dataset	2	98	October 15, 2025
Pinwheel-like 2D classes (probably due to misalignment?)	7	212	October 7, 2025
Chunky map, but decent map_sharp	16	220	September 25, 2025
I want to change pixel size before 2D/3D	3	64	September 24, 2025
RELION Polishing of EER data from CryoSPARC via csparc2star	1	460	September 22, 2025
Bump on FSC indicative of an issue with pixel sizes or beam tilt?	6	121	September 20, 2025
Processing Nanodiscs–Protein Complex Dataset	4	199	August 29, 2025
"False" resolution of local refinement	2	147	August 27, 2025
Rosenthal-Henderson plot	4	161	August 21, 2025
Can't reproduce Cryosparc results in Relion	3	204	August 20, 2025
.cs file Slot/field explanation	11	97	August 15, 2025
Normalise greyscale / intensity levels of external references	2	44	August 13, 2025
Tips for heterogeneous refinement with small, low SNR particles	8	1510	August 13, 2025
How biased is my processing pipeline?	15	343	August 12, 2025
Working with very small protein	8	2636	August 7, 2025

Case Study: End-to-end and exploratory processing of a motor-bound nucleosome (EMPIAR-10739)

education_and_advice

Hi CryoSPARC users! We are excited to share with you a new end-to-end processing case study of a nucleosome with a motor protein bound that you can follow along with! Here we guide you all the way from downloading an EM…