Stripes during Picking on Denoised DW Preview

Cryo-EM Data Processing
1

Hey,

naive question, maybe it’s a known behaviour. I have just noted the preview from template picking on denoised mcirographs show (all of the preview) regular stripes. I am attaching one. Does anybody know where it comes from? Denoised were ok.

paolo

J1723_micrograph_j1719011753505369250004063_foilhole_31660596_data_31658051_31658053_20260801_030339_eer_patch_aligned_denoisedmrc
J1723_micrograph_j1719011753505369250004063_foilhole_31660596_data_31658051_31658053_20260801_030339_eer_patch_aligned_denoisedmrc576×576 186 KB

2

Could be a gain correction artefact? Denoising doesn’t correct well for any fixed pattern noise.

3

I thought about that but power spectrum is fine. it looks I only get it after denoising.