What should I do if the protein gets denatured during cryo sample prep? I’m using a 300-mesh carbon-coated gold grid, the particles at 200kV can be seen clearly but the 2D class averages don’t have high-resolution information.
Hi Yovo, could you show the 2Ds? My first reaction when I glanced at the micrograph was “that looks great!” If the 2Ds have the expected particle shape I would not assume they’re denatured. If they’re lacking any hint of internal structure or the 3D reconstructions don’t go to hi res, something else might be causing that issue.