Hello all,
I am struggling to resolve a protein complex which consists of a homodimer (~200kDa) and a heterotrimer (~150kDa). The homodimer has a good density in 2D Classes with some preferential orirtentaion (image attached) whereas the heterotrimer complex looks smeary. As a result, the ab-initio isn’t able to resolve the heterotrimer density which is where I am currently stuck.
Please share your suggestions and experiences on how to process such dataset with one part of the complex giving smeary or continuous heterogeneity. I would highly appreciate any help.
Thanks a lot in advance!