Hi Dimitry,
Here is a tutorial for the processing of membrane proteins:
The settings explained there helped me with small non-membrane targets before. Alternatively, this issue has been discussed elsewhere. The approaches suggested in these threads also helped me to get a grip on smaller targets.
Hi @M.Grieben ,
Thanks for your post - this is an interesting case.
The parameters you chose for 2D classificaiton are appropriate for the context.
The batchsize-per-class can help here, and in fact you can turn it up to a very large value (say 1000). This will make the job slower, but it will ensure that the signal from more particles is available at each iteration of classification. You can also play with the “Initial classification uncertainty factor” - a larger value will cause classificat…
Hi [Screenshot 2020-07-31 at 11.52.36] [Screenshot 2020-07-31 at 11.54.12]
They were collected on a Talos Arctica with a K2 at 36 K magnification (1.2 A practical, 1.13 A corrected). One shot per hole on a 1.2/1.3 Au grids. Defocus was settled …
Cheers,
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