Weird streaks in the background of the reconstruction

[CryoEM1]

Related?

Streaking issues in data

Hi @zlwatson, We discussed this internally and came up with a few thoughts: Can you post the orientation distribution plots from the hetero refinement job? Perhaps these streaks are indicative of preferential orientation (though your plot (3) doesn’t show anything obvious) Can you provide an example of a micrograph? (output of the exposure curation job) Have you checked for anisotropic magnification? You can do this in cryoSPARC using the global CTF refinement job (turning everything off exce…