Hi @zlwatson,
We discussed this internally and came up with a few thoughts:
- Can you post the orientation distribution plots from the hetero refinement job? Perhaps these streaks are indicative of preferential orientation (though your plot (3) doesn’t show anything obvious)
- Can you provide an example of a micrograph? (output of the exposure curation job)
- Have you checked for anisotropic magnification? You can do this in cryoSPARC using the global CTF refinement job (turning everything off except aniso mag)
- Does this happen for one particular ribosome or for many different ones? Does the ribosome density also exhibit similar streaks?
Valentin