Volume Tools Error: Cube volumes only

Dear All,

I am practicing the " Case Study: Yeast U4/U6.U5 tri-snRNP". Based on the tutorial I got the Mask for the head (head_mask.mrc) and the Mask for the part than the head.

With “Import 3D Volumes” I input the head_mask.mrc and got its imported.mask. Then I tried the “Volume Tools” to try to dilate the imported-mask based on the tutorial.

I do not know whether I understand the tutorial correctly. For this “Volume Tools” step, for “volume” I input the refined volume from previous homogeneous refinement based on which I got the head.mrc and the corresponding imported-mask. For “mask” I input the “imported-mask” just got from head.mrc.

Unlike the tutorial, there was no “Type of volume being changed” in this recent version of cryosparc “Volume Tools”. For “Type of input volume” of “Volume Tools” I input “mask”. For “Type of output volume” of “Volume Tools” I input “mask”. I input threshold as 0.05 (I am not sure how much to input for this parameter, and please give an explanation on it, and I have also tried to input other values for this parameter, it gave the same error message as following) and “Dilation radius” as 5 based on the tutorial, and then I run this “Volume Tools”, it gave the following error:


Traceback (most recent call last):
File “cryosparc_worker/cryosparc_compute/run.py”, line 84, in cryosparc_compute.run.main
File “/home/userABC/mycryosparcv3/cryosparc_worker/cryosparc_compute/jobs/utilities/run_volume_tools.py”, line 50, in run
assert n.all(n.array(input_shape)==N_input), “Cube volumes only”
AssertionError: Cube volumes only

For “Type of input volume” of “Volume Tools” I also have input as “map”, and the “Volume Tools” gave the same error message as above.

I am looking forward to getting your reply on all issues related to this import volume error.

Best wishes,

Flemming