Volume alignment tools version compatibility issue

[olibclarke]

Hi,

Following on from here:

Adjust per-particle defocus of subparticles in volume alignment tools?

HI @rposert, This script is still very handy! However, it fails using input from volume alignment tools as of CS v4.6.2, as VAT no longer has a particles.ctf slot in the output: Traceback (most recent call last): File "adjust_defocus_subparticles.py", line 81, in <module> row["ctf/df1_A"] -= pose_recenter_vec_A[2] File "/home/user/.local/lib/python3.8/site-packages/cryosparc/row.py", line 36, in __getitem__ return self.cols[key][self.idx] KeyError: 'ctf/df1_A' VAT output (v4.2): …

What additional information can I provide to troubleshoot this @rposert? all I have done is taken an old VAT job, cloned it and ran it, and the outputs do not contain CTF slots.

Incidentally, if I create a fresh VAT job, but reconnect the same inputs as in the previous problematic case, it does generate the CTF slot correctly - so perhaps it is an issue that specifically pertains to cloning and modifying old VAT jobs?

Cheers
Oli

[wtempel]

@olibclarke

so perhaps it is an issue that specifically pertains to cloning and modifying old VAT jobs?

Currently, cloning jobs that were created in previous versions does not re-create outputs according to the current version’s job specification, which can cause the issue you observed. This is currently the expected behavior; a change to this is noted.

[olibclarke]

Hi @wtempel - thank you, but I don’t think it is exactly this. It is not that the old version of volume alignment tools generated output in a different format or anything - the original job (run with the old version) has a ctf slot in the output.

It is that then when I clone it and run it, without changing inputs, it no longer generates a CTF slot in the output. This does not seem like the expected behavior?