Small beta-barrel protein alignment

3D Refinement
21

Hi @huqi, apologies for the delay.

This usually happens because Ab Initio Reconstruction does not use all particles when producing a single class (for reasons described here). So you could

  • increase the Num particles to use parameter such that it is larger than your number of particles, or
  • use multiple classes (this might be better if you think there is still junk in your input particle stack)

I hope that helps!