Pinwheel-like 2D classes (probably due to misalignment?)

[ccgauvin94]

This looks reminiscent of a hexagonal cubic packing which is not unprecedented. Here’s a previous thread where I ran into this issue:

High particle density 2D classification

I have a very high density of particles, which I’m very excited about, but I am having some trouble finding 2D classification parameters where the classes don’t drift. For instance, here is a micrograph: [image] And the template picking is going quite well: [image] …but when I get to the 2D classification stage, things are not going very well. I think the problem is that the particle is very high symmetry, so the neighboring particles are essentially always in the same orientation, and re…

Could you post some high contrast micrographs?