Negative density value found by 3D classification

3D Classification
13

@zhangrui_wustl no worries, thanks for the honest feedback! We’re actively working on improving the job. Two thoughts:

  • As discussed in this thread, please keep in mind that the 3D Classification job uses weighted back-projection for the 3D density outputs. You may see ‘cleaner’ separation if you do a homogeneous reconstruction or refinement on the individual particle sets.

  • There is nothing directly analogous to the RELION T parameter, though you can adjust both the Target resolution (which will filter out higher frequency components), the Init structure lowpass resolution and Target class ESS fraction (which can make the initial class distributions more uniform).

Valentin