Hi All,
My refinement results are strange for particles extracted from micrographs that were Motion corrected and CTF (patch CTF estimation) determined in cryopsarc:
This same particles extracted from Warp or Relion (Gctf for Ctf), produced expected results:
Is this from Ctf issues? I used the classic mode for the cryosparc CTF patch estimation since there is an error with the new algorithm currently. Other than that, I used the default parameters in both motion correction and ctf estimation. Or maybe this is due to something else?
Thanks,
Matt