How to use selected downsampled and centered particles and original alignment box size etc.?

Sorry if this has been covered before.

To speed up 3D classification I centered a volume and particles on the region of interest and then downsampled the particles (real and Fourier space cropping) . I then ran 3D classification with the downsampled particles. The question now is how to make a reconstruction (homogeneous reconstruction) at the original resolution and box size but only of the particles of one of the classes from the 3D classification.

The workflow so far looks like;

J1 Vol & Particles after previous alignment → J2 Volume alignment tool (centering) → J3 Particle Downsampling with the option Recenter Using Alignment Shifts ON → J3 3D classification

?? → J4 homogeneus reconstruction using alignments box and pixel size of J1 but using only particles of class 1 from J3 3D classification

Thanks

H.

Use the expanded inputs interface.

In your example, replace the “particle.blob” slot of J4 with the one from J1 (via drag and drop from the outputs tab of J1), then reconstruct.

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Thanks it makes sense. A related question: is there a way to list an expanded view ion the input parameters of a completed job (eg. in the input parameters tab) This will be useful to verify whether the particle blob entry was replaced and what particular blob particles were given as input.

Thanks again

H.

You can click “show slots”, but I agree it is not ideal at present, improvements requested here: Visually distinguish inputs that have been modified

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Thank you that is what I was looking for.

H.