Sorry if this has been covered before.
To speed up 3D classification I centered a volume and particles on the region of interest and then downsampled the particles (real and Fourier space cropping) . I then ran 3D classification with the downsampled particles. The question now is how to make a reconstruction (homogeneous reconstruction) at the original resolution and box size but only of the particles of one of the classes from the 3D classification.
The workflow so far looks like;
J1 Vol & Particles after previous alignment → J2 Volume alignment tool (centering) → J3 Particle Downsampling with the option Recenter Using Alignment Shifts ON → J3 3D classification
?? → J4 homogeneus reconstruction using alignments box and pixel size of J1 but using only particles of class 1 from J3 3D classification
Thanks
H.