Hi, the homogeneous refinement failed when I extract particles using original box size (752 pixel) without downsample, The pixel size of the micrograph is 0.653 A. The error message shown below:
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Hi,
The text of the error message is below:
Traceback (most recent call last):
File “cryosparc_master/cryosparc_compute/run.py”, line 96, in cryosparc_compute.run.main
File “cryosparc_master/cryosparc_compute/jobs/refine/newrun.py”, line 348, in cryosparc_compute.jobs.refine.newrun.run_homo_refine
File “cryosparc_master/cryosparc_compute/engine/engine.py”, line 941, in cryosparc_compute.engine.engine.process
ZeroDivisionError: division by zero
All the parameters are default setting.
Our team looked into this error and concluded that the GPU memory in this case is too small to accommodate the relatively large box and other inputs.
The team’s recommendation in this case is to try this job after disabling Re-estimate greyscale level of input reference. Does this help?