Dear cryoSPARC community,
I am wondering if I am doing something fundamentally wrong when I am merging particles from 2 datasets.
I have two datasets (dataset A ~109K and B 280K particles) that were collected at the same pixel size. I’ve processed the data individually and I got maps at ~4.5Å and ~3.5Å for each dataset.
I re-extracted particles at the same box size and tried to combine them by simply dragging particles from both data sets as input to 2D classification.
Based on the output (2D class representative picture attached) it doesn’t look like the particles are being merged – The contrast is very different. Similar results was observed when I run an ab initio with 2 classes.
Any thoughts, ideas, on the matter would be much appreciated