Dark 2D Classes after Min over Scale

[chew0357]

I am facing a similar issue of light vs dark 2D classes upon merging particles from two datasets, have just posted herein seeking for advice please:

Help with merging particles from 2 datasets

Dear all, I have two datasets of the same protein sample collected on two different dates 13 days apart with the same microscope and settings (apix, dose). I processed the data on CryoSPARCv4.2.1 in two separate workspaces from motioncorr to 3d refinement before trying to merge the two sets of particles. Both sets of particles (same box size) were exported and reimported to match the pixel size (prior error mismatch in pixel size up to 4 decimal places). However the 2D classes seem to show op…

hoping to understand what I might doing wrong or how to fix