GSFSC curve bilobed

Troubleshooting
1

The FSC curves I have seen so far typically show a smooth fall. But for this dataset, I am getting a bilobed GSFSC curve while running a homogenous refinement. What possibly have I been doing wrong?

Thanks,
SS

2

Hi @ss4858, are you working with a membrane protein or highly symmetric structure?

3

Also, are the automatic masks reasonable looking?

4

Were your micrographs crowded with particles and they were picked too close to each other? This could mean, among other things, that you have several particles with overlapping boxes in your dataset.

5