Filament tracer fail

Helical Processing
1

error below. Filament tracer in 3.3.1.

No matter what options I try I cannot get it to work. I use the ctf estimates from patch ctf. I try without template and with template. Nothing works.

error:
[CPU: 535.2 MB] Traceback (most recent call last):
File “cryosparc_worker/cryosparc_compute/run.py”, line 85, in cryosparc_compute.run.main
File “cryosparc_worker/cryosparc_compute/jobs/template_picker_gpu/run.py”, line 61, in cryosparc_compute.jobs.template_picker_gpu.run.run
File “cryosparc_worker/cryosparc_compute/jobs/template_picker_gpu/run.py”, line 250, in cryosparc_compute.jobs.template_picker_gpu.run.do_pick
File “cryosparc_worker/cryosparc_compute/jobs/template_picker_gpu/run.py”, line 441, in cryosparc_compute.jobs.template_picker_gpu.run.do_pick
File “cryosparc_worker/cryosparc_compute/jobs/template_picker_gpu/filament_tracer.py”, line 548, in cryosparc_compute.jobs.template_picker_gpu.filament_tracer.FilamentTracer.trace_all_filaments
AttributeError: ‘float’ object has no attribute ‘astype’

2

Hi @orangeboomerang,

Thanks for reporting, and apologies that you’re facing this problem. I believe this occurs when only one pick is found on a filament, which could occur if the separation distance parameter is set too large (e.g. this similar post on this issue). Regardless, this should be fixed and thank you for bringing it to our attention; we have it tracked for a patch :slight_smile:

Best,
Michael

1 Like
3

Hi @orangeboomerang,

We have released cryoSPARC v3.3.2 with a fix for this AttributeError. Please let us know if the issue is resolved when running the Filament tracer job on the newest version!

Best,
Michael

1 Like