Extraction of same particle subset from different patch motion correction jobs

[kplkmrgoutam]

Hi all,
I wanted to ask if it is possible to extract the same set of particles from different (patch) motion correction jobs in cryoSPARC v3. I have a particle subset extracted and cleaned in v2.12, I want to extract it from job motion-corrected in v3 (same set of original micrographs). When I try to extract the particles the jobs give “no particle” output in v3, although it completes without showing any error. Is there anything I can change to make it work?
Best,
Kapil

[nfrasser]

Hi @kplkmrgoutam, see my post here for details:

Extract particles from different Patch Motion outputs

Hi @jianhaoc, yes you can do this but it’s a bit complicated. The particle locations are tied to the path of the motion-corrected micrographs on disk. That means you’ll have to duplicate the particle locations and manually modify the “path” component of the location. Here’s how to do that. Copy the micrograph_blob path from the J2 outputs as indicated. Note this down somewhere [Copy micrograph_blob path] Export the job you used to generate the particle locations from the micrographs in J1…