I’m sure many of you have appreciated cryoSPARC’s excellent semi-manual template picking feature. However, a frustration of mine is the lack of a fully automated picker using e.g. a Gaussian blob. I’m sure the team has something like this in the works, but here is my solution for the meantime.
Simply import the attached image - a circular blob - as a template!
This approach has worked for every negative stain and cryo dataset I’ve tested so far. The diameter is controlled by setting the pixel size during import. For example, for a 160 px diameter template, the pixel size can be set to 5 A (the image is 32 px across).
PS the source of the image is an empty class from a negative stain project.
PPS if someone has a better single template to share, please do!