Hello Oli @olibclarke,
Are you sure the ab initio is a correct solution?
Good question – from data that I have – perhaps, yes. But after you mentioned that – I am intensively checking and remastering it. Thank you for your remarks.
To me it looks a little like one of the failure modes we often see, described here: "side view prior" for ab initio?
Did you find a solution at the end? As I understood, it happened due to limited views. Did the re-picking step help?
It doesn’t seem to have many high resolution features - can you clearly see secondary structure in the ab initio, and what settings are you using for ab initio?
Well, not really – I see a bit in 2D classes. The setting is the default ones + 0.01 for the Fourier redius step (I found it worked better for me)
Are clear secondary structure features present in the 2D classes?
These are the ones.
Thank you.
Kind regards,
Dmitry