Advice for 3DVA of glycoprotein bending and breathing

Hi @ArmandoPach and @olibclarke,
I have since tried to increase the box size but unfortunately got worse results as explained in this post Why does smaller box size give better resolution? - CryoSPARC Parameters - cryoSPARC Discuss.
I also thought the box was a bit tight but the limit seems to be 256pix for the monomer and 360pix for the dimer. Thanks for the advice though!

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