wwPDB Submission Form: Angle assignment type

Dear colleagues,

Quick question - what is the “angle assignment type” used

for a) Ab-initio
b) refinement

in cryosparc algorithms?

Thank you

Sincerely,
Dmitry

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Maximum likelihood for refinement, I believe this is also the best choice for ab-initio, but would you mind listing the available options as I can’t recall off the top of my head.

(Note: @Dmitry is referring to the provided options in the wwPDB deposition server).

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Dear Daniel @DanielAsarnow,

Thank you for your answer.

So the options from the PDB deposition site are:

  • angular reconstitution
  • common line
  • maximum likelihood
  • not applicable
  • ohter
  • projection matching
    -random assignment

Thank you

Sincerely,
Dmitry

Hi @Dmitry, @DanielAsarnow is correct - Maximum Likelihood is correct for both ab-initio and refinement in cryoSPARC!

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Might be helpful to have a cryosparc guide for EMDB submission - this (and related queries re the meaning of parameters requested by the EMDB) are not uncommon queries

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