I combined two sets of down-sample particle stacks (from two different datasets) for a NU-refinment. Then I noticed that the FSC did not drop to 0 at Nyquist frequency. So I checked duplicated items using job “Remove Duplicates”.
Either of stacks shares few duplicated items (<100) while the combined set gives me a number of ~70000. And I am pretty sure that these two stacks has no intersection.
May be the same down-sample file name of batch_xxx_downsample.mrc do the wrong thing?
I did make a mistake to import duplicated movies at first. So I had two copies of my first dataset with different uids and I didn’t realize it until I checked all involved job’s parents.
Sorry to bother you.