Please post the log contents seen in the screen shot as text so that forum visitors with a similar problem may easily find this topic.
Please can you also post a screenshot of the Inputs and Parameters tab.
If you are referring to cryosparcw connect ..., the answer is no:
cryosparcw connect ... needs to be run only when
- a new worker needs to be connected to the CryoSPARC instance or
- the configuration of an existing worker has changed
Worker configuration information is stored in the CryoSPARC database and persists between restarts of the master services or the master computer.