Why the X-axis of the GSFSC curve is only 3A in CryoSPARC's Homo Refine? And how can I change it to 2 or 1 A?

Troubleshooting
1

Hi,

I am new to CryoSPARC and have a basic question. Why is the X-axis of my GSFSC curve limited to 3 Å, making it impossible to see values where the Y-axis drops to 0? Which parameter should I adjust when setting up the Homogeneous Refinement job to change the range of the X-axis?

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Thank you so much!

Best,
Junqing

2

@jkang3 What is the pixel size (in Å) of the input particles?

3

Hi,
It is 1.08

Thank you

4

I think you either down sample your particles, or super resolution but not type pix size accordingly.

5

Thank you so much for your reply.
You mean the parameter of extraction is not appropriate?

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What number should I input for a good GSFSC curve?

Thank you for your help.

Best,
Junqing

6

you binned your data 1.3-fold (500/384), so your pixel size is now 1.4A, corresponding to the Nyquist limit 2.8, which is where the X axis ends on your plot.

2 Likes
7

Now I know, thank you so much~

Best,
Junqing

1 Like