Hi,
I plotted the 3D maps and I also see these beautiful graphs of the 3D structure, but I am having hard time reding these curves. Could anyone please let me know what are thesee different kinds of Angosterm curves?
And what does GSFSC resolution mean?
Thanks in advance!
HI Ashwin, it is more or less covered here: Loose mask in non-uniform refinement - #2 by mmclean
GS-FSC refers to the so called “gold standard” Fourier Shell Correlation curve, in which the two half sets of particles are processed independently, and then a correlation is calculated between the two half sets of data using a threshold of FSC=0.143 (the so-called gold standard criterion).
Cheers
Oli