What can I learn from this cFAR curve

Cryo-EM Data Processing
22

{‘_id’: ‘691d0ced4bf33e3c42396dfa’, ‘errors_run’: , ‘job_type’: ‘import_movies’, ‘params_spec’: {‘accel_kv’: {‘value’: 300}, ‘blob_paths’: {‘value’: ‘/data/CryoEM/data_from_KRIOS_NYC/frames/n25nov12f/rawdata/*eer’}, ‘cs_mm’: {‘value’: 2.7}, ‘gainref_flip_y’: {‘value’: True}, ‘gainref_path’: {‘value’: ‘/data/CryoEM/data_from_KRIOS_NYC/frames/n25nov12f/rawdata/references/n25nov06e_06130458_00_4096x4096_norm_0_mod.mrc’}, ‘mov_cut_prefix_xml’: {‘value’: 0}, ‘mov_cut_suffix_xml’: {‘value’: 11}, ‘parse_xml_files’: {‘value’: True}, ‘psize_A’: {‘value’: 0.73}, ‘total_dose_e_per_A2’: {‘value’: 46.7}, ‘xml_cut_prefix_xml’: {‘value’: 0}, ‘xml_paths’: {‘value’: ‘/data/CryoEM/data_from_KRIOS_NYC/frames/n25nov12f/rawdata/*xml’}}, ‘project_uid’: ‘P24’, ‘started_at’: ‘Sun, 23 Nov 2025 06:34:30 GMT’, ‘status’: ‘completed’, ‘uid’: ‘J1497’, ‘version’: ‘v4.6.2’}

[Sun, 23 Nov 2025 06:34:34 GMT] [CPU RAM used: 320 MB] Importing movies from /data/CryoEM/data_from_KRIOS_NYC/frames/n25nov12f/rawdata/*eer
[Sun, 23 Nov 2025 06:34:34 GMT] [CPU RAM used: 322 MB] Importing 6275 files
[Sun, 23 Nov 2025 06:34:35 GMT] [CPU RAM used: 326 MB] Import paths were unique at level -1
[Sun, 23 Nov 2025 06:34:35 GMT] [CPU RAM used: 326 MB] Importing 6276 files
[Sun, 23 Nov 2025 06:34:35 GMT] [CPU RAM used: 326 MB] ‘Skip Header Check’ parameter enabled, checking first header only
[Sun, 23 Nov 2025 06:34:35 GMT] [CPU RAM used: 351 MB] EER data imported with upsampling factor 2.00. Old pixel size: 0.7300 new pixel size 0.3650
[Sun, 23 Nov 2025 06:34:35 GMT] [CPU RAM used: 351 MB] Reading headers of gain reference file /data/CryoEM/data_from_KRIOS_NYC/frames/n25nov12f/rawdata/references/n25nov06e_06130458_00_4096x4096_norm_0_mod.mrc
[Sun, 23 Nov 2025 06:34:35 GMT] [CPU RAM used: 351 MB] Importing XML files from: /data/CryoEM/data_from_KRIOS_NYC/frames/n25nov12f/rawdata/*xml
[Sun, 23 Nov 2025 06:34:35 GMT] [CPU RAM used: 353 MB] Example movie/mic name for XML correspondence, after applying prefix/suffix cut:
n25nov12f_00001eer
[Sun, 23 Nov 2025 06:34:35 GMT] [CPU RAM used: 353 MB] Example XML name for movie/mic correspondence, after applying prefix/suffix cut:
n25nov12f_00001eer
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 353 MB] There were 0 exposure(s) with non-unique XML correspondences. These were imported with no beam shift values.
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 353 MB] There is a total of 0 exposure(s) with missing beam shifts.
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] Done importing.
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] --------------------------------------------------------------
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] ===========================================================
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] Loaded 6275 movies.
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] Common fields:
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] mscope_params/accel_kv : {300.0}
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] mscope_params/cs_mm : {2.7}
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] mscope_params/total_dose_e_per_A2 : {46.7}
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] mscope_params/exp_group_id : {67}
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] mscope_params/phase_plate : {0}
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] mscope_params/neg_stain : {0}
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] movie_blob/psize_A : {0.365}
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] movie_blob/shape : [ 40 8192 8192]
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] movie_blob/is_gain_corrected : {0}
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] ===========================================================
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] Making example plots. Exposures will be displayed without defect correction.
[Sun, 23 Nov 2025 06:35:38 GMT] [CPU RAM used: 362 MB] Reading file…

23

Thanks @F-GST for posting the job information. Please can you also post any plots or log excerpts that led you to conclude

24

I am sorry I dont have enough experience, but based on the above comments , I was expecting to see this plot , which I didnt find

25

The plot should visible near the top of the Event Log. To scroll back to the top of the Event Log, you may need to push the Show from top near the upper left corner of the UI:

image
image386×298 17.3 KB

The plot should appear just below the line that includes

There is a total of 0 exposure(s) with missing beam shifts.

image
image1362×236 40.2 KB

What is shown just below the line

in the event log of your job P24-J1497?

26

image
image630×462 32.7 KB

this is my plot now I see it …

but still I am having issue, when I start with the movies and perform Patchmotion correction followed by patch CTF, I notice a bright dot in the middle of the image -

image
image720×511 85.6 KB
this is my parameters for the

image
image1316×687 101 KB

and I tried to also turn on the option of flipping the gain reference in X direction and same issue

27

It doesn’t look to me that there are resolutions of less than 3A in that set. That makes it unlikely that you get a much better resolution from this set of micrographs (if I understand it correctly, I am rather new to this, so I might not…)