I am having issues using an imported particle stack.
Here’s the general workflow:
Preprocessing and 2D classification in CryoSPARC
make star file using pyem
run 3D classification in Relion
Subset selection of the best class
Import particle stack to cryoSPARC: import only the star file, connect to the original preprocessed micrographs
So far so good, to now use this subset of particles for homogeneous refinement, I connect the imported particles, then add the blob from the original cryoSPARC extraction job, connect initial model and run.