I would like to use LocScale to sharpen my cryosparc map from local refinement using my current refined model as a reference. An unfiltered, unsharpened map is required by LocScale. From previous posts on this forum I understand that the half-maps are unfiltered. Is the volume_map.mrc file also unfiltered?
I just tried LocScale using the volume_map.mrc file from cryosparc and it gave much poorer features in the output LocScale map than when I run it with a volume_map_half_A.mrc file. Does that mean the former is filtered? If so, how can I generate an unfiltered full map?