Hi,
I have installed the latest version of Topaz from Tristan’s GitHub page using the instructions he provided. Then, I followed your instructions to make the exe visible to cryosparc. Having done this, when I run the program from within cryosparc, it is executed successfully upto a certain stage (see below for the log) and then the process does not appear to progress beyond the THCudaCheck error. Can you help me understand how this problem can be circumvented?
[CPU: 166.7 MB] Starting Topaz process using version 0.2.3...
[CPU: 166.8 MB] Random seed used is 1328817122
[CPU: 166.8 MB] --------------------------------------------------------------
[CPU: 166.8 MB] Starting preprocessing...
[CPU: 166.8 MB] Starting micrograph preprocessing by running command /path/to/topaz/bin/topaz preprocess --scale 16 --niters 200 --num-workers 8 -o /path/to/preprocessed [MICROGRAPH PATHS EXCLUDED FOR LEGIBILITY]
[CPU: 166.8 MB] Preprocessing over 4 processes...
[CPU: 166.9 MB] Inverting negative staining...
[CPU: 166.9 MB] Inverting negative staining complete.
[CPU: 166.9 MB] Micrograph preprocessing command complete.
[CPU: 166.9 MB] Starting particle pick preprocessing by running command /path/to/topaz convert --down-scale 16 --threshold 0 -o /path/to/topaz_particles_processed.txt /path/to/topaz_particles_raw.txt
[CPU: 166.9 MB] Particle pick preprocessing command complete.
[CPU: 166.9 MB] Preprocessing done in 47.271s.
[CPU: 166.9 MB] --------------------------------------------------------------
[CPU: 166.9 MB] Starting train-test splitting...
[CPU: 166.9 MB] Starting dataset splitting by running command /path/to/topaz train_test_split --number 19 --seed 1328817122 --image-dir /path/to/topaz_particles_processed.txt
[CPU: 166.9 MB] # splitting 97 micrographs with 15403 labeled particles into 78 train and 19 test micrographs
[CPU: 166.9 MB] # writing: /path/to/topaz_particles_processed_train.txt
[CPU: 166.9 MB] # writing: /path/to/opaz_particles_processed_test.txt
[CPU: 166.9 MB] # writing: /path/to/image_list_train.txt
[CPU: 166.9 MB] # writing: /path/to/image_list_test.txt
[CPU: 166.9 MB]
Dataset splitting command complete.
[CPU: 166.9 MB] Train-test splitting done in 18.990s.
[CPU: 166.9 MB] --------------------------------------------------------------
[CPU: 166.9 MB] Starting training...
[CPU: 166.9 MB] Starting training by running command /path/to/topaz/bin/topaz train --train-images path/to/image_list_train.txt --train-targets /path/totopaz_particles_processed_train.txt --test-images path/to/image_list_test.txt --test-targets/path/to/topaz_particles_processed_test.txt --num-particles 100 --learning-rate 0.0002 --minibatch-size 128 --num-epochs 10 --method GE-binomial --slack -1 --autoencoder 0 --l2 0.0 --minibatch-balance 0.0625 --epoch-size 5000 --model resnet8 --units 32 --dropout 0.0 --bn on --unit-scaling 2 --ngf 32 --num-workers 8 --cross-validation-seed 1328817122 --device 0 --no-pretrained --save-prefix=/path/to/models/model -o /path/to/train_test_curve.txt
[CPU: 166.9 MB] # Loading model: resnet8
[CPU: 166.9 MB] # Model parameters: units=32, dropout=0.0, bn=on
[CPU: 166.9 MB] # Receptive field: 71
[CPU: 166.9 MB] # Using device=0 with cuda=True
[CPU: 166.9 MB] # Loaded 78 training micrographs with 12548 labeled particles
[CPU: 166.9 MB] # Loaded 19 test micrographs with 2855 labeled particles
[CPU: 166.9 MB] # source split p_observed num_positive_regions total_regions
[CPU: 166.9 MB] # 0 train 0.0845 363892 4306302
[CPU: 166.9 MB] # 0 test 0.0789 82795 1048971
[CPU: 166.9 MB] # Specified expected number of particle per micrograph = 100.0
[CPU: 166.9 MB] # With radius = 3
[CPU: 166.9 MB] # Setting pi = 0.0525276675904
[CPU: 166.9 MB] WARNING: pi=0.0525276675904 but the observed fraction of positives is 0.084502201657 and method is set to GE-binomial.
[CPU: 166.9 MB] WARNING: setting method to PN with pi=0.084502201657 instead.
[CPU: 166.9 MB] WARNING: if you meant to use GE-binomial, please set pi > 0.084502201657.
[CPU: 166.9 MB] # minibatch_size=128, epoch_size=5000, num_epochs=10
[CPU: 166.9 MB] THCudaCheck FAIL file=/opt/conda/conda-bld/pytorch_1544084119927/work/aten/src/THC/THCGeneral.cpp line=405 error=11 : invalid argument
Looking forward to hearing from you.
Best,
Pranav
