I have noticed with more than 10 different datasets of virus samples (I symmetry) that both
Ab-initio reconstruction and Heterogeneous Refinement never result in proper 3D densities
when I symmetry is not enforced. Instead, we always get donut/ring-shaped volumes. Enforcing
I symmetry however does result in correct volumes. I am afraid that enforcing symmetry at this
early stage of processing leads to sub-optimal final results. Also, we do try all the tips & tricks
recommended on this board and elsewhere for dealing with symmetries (e.g. increasing batch
size or initial/final resolution etc.), but so far this has not solved our issues in any of our datasets.
I am wondering if there are any plans to introduce new features or optimize workflows for
dealing with high symmetry particles?