Sort particles based on particle scale factor

Hi,
My homogeneous refinement job shows a bimodal distribution in the per-particle scale factor, despite achieving a score of 0.944. I attempted multiple rounds of 3D classification, but they were not helpful. Does CryoSPARC have a job that can separate particles based on the scale factor?

You can do this using the 3D Flex data prep module - not the most elegant but it works…

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Thanks! It seems to have worked—I can clearly see the results in the per-particle scale plot. However, I didn’t notice any improvement in the resolution. :stuck_out_tongue_winking_eye: That’s a whole other issue.

Appreciate your quick help!

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Hi @Ravi.Thakkar369!
This is now an option in the new “Subset particles by Statistic” job in CryoSPARC v4.7.
– Katherine

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