Small membrane proteins: 2D classification and ab-initio reconstruction

[rkhayat]

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Cannot align small protein complex particles

Hi All, I’ve collected a dataset of a small protein complex (~50 kDa) with surprisingly (to me at least) good contrast, but cannot get them to align in either 2D or 3D. The particles are fundamentally mostly beta propellers that are ~45A in diameter. I’m attaching a 2.5 um defocus micrograph for reference. A bit dense yes, but not too dissimilar to some of the published micrographs that yield high resolution structures. [example] The images were collected using on a Titan Krios with a K3 and…