Rosenthal-Henderson plot

Cryo-EM Data Processing
1

Hi CryoSparc community,

I’m looking for advice on the best practices to generate a Rosenthal-Henderson plot in CryoSPARC. Is the current recommended approach to run multiple refinement jobs with varying numbers of particles, or is there a more streamlined method?

Thanks in advance for any guidance!
Yue

2

Hi Yue,

Are you trying to just figure out how many particles are sufficient to get a certain resolution (or figure out if your resolution has plateaued with your current number of particles)? If so you can run a Reslog analysis job and it will generate the plots for you. See the job details here.

1 Like
3

Thanks a lot @tlevitz ! This looks like exactly what i need… appreciate your help : )

1 Like
4

Also see ResLog error bars? - #2 by rwaldo and [3dem] B-factors

3 Likes
5

Thanks @WimJHH these are very helpful information!