Ring like artifacts

[clayton]

Hi all,

I’m working on a protein with an approximate molecular weight of 150 kDa. Despite collecting a large dataset, the EM map resolution appears to be stuck at around 6 Å.

One of the main issues I’m encountering is the appearance of ring-like artifacts around the map, and it also seems to be over-sharpened.

Could anyone suggest how to minimize or avoid these artifacts during map reconstruction or post-processing?

I’m attaching a few EM map snapshots for your reference.

Thanks in advance for your support!

[olibclarke]

Classic example of overfitting. You will need to provide more details about your processing workflow to help figure out a cause/solution.

[Mark-A-Nakasone]

@clayton did you play with dynamic masking in homo or NU refine ?

How are the cFAR values ?