Resolutions jumping to high resolutions after Non-uniform refinement

[Jinseo]

Hi Everyone,

I am wondering while processing my data.
I stuck my Homogeneous Refinements at around 6.4~6.5A
But after homo, I tried to do non-uniform.
Most of non-uniform reached around 5.1~5.2A and showed better features of density maps like less junk particles, stronger density parts.
Is it reasonable that kind of resolution jumping between Homo- and Non-uniform refinements?

Here are my FSC curves.

Homo -

Non-uniform

Please give any advices or validations.

Thanks

Jinseo

[olibclarke]

Hi Jinseo,

Yes, this is expected in favourable cases.

Oli

[Jinseo]

Hi Oli

Thanks for answer.
I was concerning about that can be fake.

Jinseo