“Remove Duplicates” doesn't work after 3DVA

3D Variability Analysis
1

Hello, every Cryo-EM expert

I run 3DVA with symmetry expanded particles and displayed the result with the cluster mode (6 clusters). I selected 5 of clusters to run ‘remove duplicates’ but the particles were greater than that should be. I did change the minimum separation distance to 0 and shift key to ‘none’. If I use all of particles in all clusters to run the job, there would be no problem. I don’t know why this happened. I am using CS4.6.2, BTW. Thank you so much for your kind suggestions in advance!

2

If you set minimum distance to zero, it defines particles which are 0.1Å dislocated as unique. Set Minimum separation distance to, e.g.; 50% of your particle diameter and see what effect that has.