Relion_reconstruct failed after re-extract refined particles from cryosparc

[cryofun]

How different is your relion refined map from the cryosparc refined map?

If you import those particles to cryosparc and refine against that cryosparc map, do you reach the same result?

I have a similar issue

Cannot replicate refinement from cryosparc after csparc2star

I am encountering a strange issue after running csparc2star. My goal is to extract particles without normalization after running standard processing in cryosparc. Since cryosparc cannot do this, I must extract in relion. I am not extracting without normalization, yet, just testing the pipeline with normal settings that should be consistent with cryosparc processing. My current issue is that when I run csparc2star.py, extract particles in relion, then import those particles to cryosparc and run …