Hello,
We try to extract particles in relion (v. 4.0) using home-refined particles from cryosparc (v.4.4.1) and attempted to generate the same 3D map using relion_reconstruct.
. pyem (version 0.5)
. csparc2star.py xxx_particles.cs xx_passthrough_particles.cs xxxx.star
. using vi commend, change ‘_rlnMicrographName’ to micrograph names in MotionCorr/jobxx/Micrographs/xxxx.tif
. extract particles with an option of re-extract refined particles
. relion_reconstruct --i Extract/jobxx/particles.star --o Extract/jobxx/map.mrc --ctf --angpix xx
particle extraction seems to work (based on 2D classification), but relion_reconstruct failed to generate the same 3D map derived from cryosparc homo-refine.
it seems like ‘_rlnAngleRot’, ‘_rlnAngleTilt’, ‘_rlnAnglePsi’ info were transferred to .star file.
Does anyone know what the problem is?
Uhnsoo