3D-VA runs out of GPU memory when I use large particles (512px). As a consequence, I want to downsample my particles and rerun refinement, prior to 3D-VA.
I downsampled my particles (from a refinement job) from 512 to 256 using the downsample job type, then reran refinement, giving the weird FSC attached with resolution of 10 Å. The initial res prior to downsampling was 2.8 Å. Starting the refinement from an ab initio model generated from the downsampled particles gave the same result.
Any ideas as to what is going on here and how to fix it much appreciated!