I’d try doing the reinstall script again so it re-writes/refreshes - if the PATH was missing after it was re-added.
When you do the CUDA Installation you’re supposed to set the PATH / LD_LIBRARY_PATH environmental variables (it’s listed as an instruction after CUDA install finishes – on the terminal screen)
may want to set LB_LIBRARY_PATH also (not sure if Cryosparc uses that variable, but it might)
export LD_LIBRARY_PATH=$LD_LIBRARY_PATH=/usr/local/cuda/lib for example.
If you’d like to reinstall cryoSPARC, you can delete cryosparc2_master and cryosparc2_worker folders, and start again (after re-extracting the tar archives).