Release Notes
Added
- Pre-processing jobs no longer fail immediately when a child process encounters a segmentation fault
- Patch Motion jobs now use an improved IO library, providing substantial speedups on some cluster file systems
- Blob Picker Tuner job now reports progress during optimization
- Validation (FSC) job now takes in an optional
Override Pixel Size (A)parameter to allow adjusting the final pixel size and resolution estimate if incorrect pixel size was specified during processing - Align 3D job now takes in particle stack(s) and rotates/shifts the alignments of the particles to match the rotation and shift applied to the volumes during 3D alignment
- Project deletion (and other long running actions) are now decoupled from instance-wide locks, allowing a cryoSPARC instance to continue to perform unrelated actions while a deletion is ongoing
Updated
- Outputs in the Sharpening Tools job are now separated into two output groups and can be directly connected to downstream Volume Tools jobs
- Warnings in the Import Particles job’s Overview tab are now more visible when fields are missing in the imported metadata files
- Warnings in the Check For Corrupt Particles job’s Overview tab are now more visible when corrupt files are found
- The Local Refinement (NEW!) job now explicitly requires an input mask
- Blob picker output images now show more contrast for negative stain data
Fixed
- Allow clearing Extract Particles jobs created before the previous patch
- Prevent Motion Correction job failure due to low-level memory error from corrupt TIFF files
- Corrected stdout/stderr redirect options in
cryosparcm cluster examplescripts - Manual picker and Inspect picks jobs no longer overly downsample rectangular (K3) images
- Job status timestamps are now correctly cleared when a job is cleared
- Patch CTF job no longer fails with data from spherical aberration-corrected microscopes
Installation
See installation instructions here (cryoSPARC v3.3.2 required):
https://guide.cryosparc.com/setup-configuration-and-management/software-updates#apply-patches